Research Only Healing & Recovery

TB-500

also known as: Thymosin beta-4 fragment, Tβ4 fragment, TMSB4X peptide

A synthetic fragment of thymosin β4 studied in animal models for actin-binding-mediated tissue repair, particularly in cardiac and soft-tissue injury.

A shortened synthetic analog of the natural regenerative peptide thymosin β4 (Tβ4), sharing its actin-binding motif but differing in structure and systemic activity.

Mechanism of action

Binds G-actin and modulates cytoskeletal dynamics, cell migration, and angiogenesis. Preclinical studies show effects on endothelial cell migration, tissue revascularization, and inflammation modulation. Often combined with BPC-157 in community protocols for soft-tissue injuries despite absence of clinical trials.

Primary uses

Soft tissue injury (animal models)
Cardiac repair (preclinical Tβ4 studies)
Corneal wound healing (Tβ4 clinical trials)
Equine veterinary use

Typical dosing

2–2.5 mg 1–2x weekly loading, then weekly maintenance (subcutaneous)

Community dosing. No human clinical trial has established a TB-500-specific dose.

Regulatory status

Not approved by any regulatory body for any indication. Full thymosin β4 has been studied in FDA-registered trials for dry eye (Lacripep/RGN-259) and heart failure, but TB-500 as marketed in the peptide community is the short synthetic fragment, not full Tβ4.

References

[pubmed] Goldstein AL, et al. "Thymosin β4: a multi-functional regenerative peptide." Expert Opin Biol Ther, 2012;12:37-51.
[clinical-trial] Dunn SP, et al. "Evaluation of RGN-259 ophthalmic solution (Lacripep, thymosin β4) for dry eye." ClinicalTrials.gov NCT03784768.
[review] Crockford D, et al. "Review of the clinical pharmacology of thymosin β4 and utility as a therapeutic." Ann N Y Acad Sci, 2010;1194:179-189.

Related peptides

BPC-157

A synthetic pentadecapeptide from gastric protein, widely studied in animal models for tendon, ligament, and gastrointestinal healing.

GHK-Cu

A naturally occurring human tripeptide-copper complex with extensive topical cosmetic evidence and strong in-vitro data on collagen synthesis, angiogenesis, and anti-inflammatory signaling.

Disclaimer

This entry is for educational purposes only and does not constitute medical advice. Dosing information reflects published regulatory or research data and is not a recommendation. Many compounds described here are not approved for human use in the United States. Consult a licensed medical professional before considering any peptide therapy.